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A: Doctor Climax was filmed in the year 2023, with production taking place over several months to accurately depict the 1970s setting of Bangkok. The filming involved a combination of on-site shooting and post-production visual effects to maintain the shows period look. Q: How did the production team reproduce a 1970s environment in The PD-D/EXK phosphodiesterases span over 21,900 proteins, which can be classified into 121 groups of various families. Eleven of them, including DUF4420, DUF3883, DUF4263, COG5482, COG1395, Tsp45I, HaeII, Eco47II, ScaI, HpaII and Replic_Relax, are newly assigned to the PD-D/EXK superfamily. Some groups of PD-D/EXK proteins are present in Background: The PD-D/EXK nuclease superfamily, initially identified in type II restriction endonucleases and later in many enzymes involved in DNA recombination and repair, is one of the most challenging targets for protein sequence analysis and structure prediction. Typically, the sequence similarity between these proteins is so low, that most of the relationships between known members of Background: PD-D/EXK nucleases constitute a large and highly diverse superfamily of enzymes that display little sequence similarity despite retaining a common core fold and a few critical active site residues. This makes identification of new PD-D/EXK nuclease families a challenging task as they usually escape detection with standard sequence-based methods. The active sites of MgII-dependent nucleases feature a cluster of conserved charged residues which includes both acidic Asp and Glu and basic Lys side chains. In restriction enzymes, these side chains are part of the conserved PD D/EXK functional sequence motif which has been implicated as being important in metal ion binding and catalytic steps. Recent work revealing the unusual XK-related protein. Members of this family comprise various XK-related proteins, that